June 30

 June 30, 1956: The last passenger train makes its final run on the Rochester Subway. The subway began operations in 1927 and limited freight service continued to Gannett Newspapers until 1996.

Sources and Further Reading:

1. Vintage Views: Rochester's History: an Illustrated Timeline; "End of the Line" 
2. Wikipedia: Rochester Subway
3. Rochester Ponders Future of Once Vibrant Subway; Mercer, Laurie; Oct 31, 2007